The Hepcidin ELISA test is an enzyme immunoassay that measures Hepcidin levels in serum. This immunoassay measures human Hepcidin in serum, plasma, cell culture supernates and urine. It takes approximately 4.5 hours to complete. It contains synthetic Hepcidin as well as antibodies against synthetic Hepcidin.
Hepcidin ELISA equipment was able to accurately quantify synthetic and naturally occurring Hepcidin. It takes approximately 4.5 to 5 hours to complete. It contains synthetic Hepcidin as well as antibodies against synthetic Hepcidin. This immunoassay was able to accurately quantify synthetic and naturally occurring Hepcidin.
The Human Hepcidin ELISA Kit allows you to test for Human Hepc using the following samples like plasma, serum, blood, plasma, cell cultures supernatant, and other supernatants or tissues. The ELISA kits can be used to measure analytes in a variety of samples. ELISA Genie has developed a range of reliable, sensitive and fast ELISA kits to meet the demands of today's scientists.
Each kit includes highly specific antibodies pre-coated on a 96-well microtiter plate. We understand that speed is important at ELISA. This kit has a fast protocol that will allow you to get your results quickly. Once you have prepared your samples and prepared the standards, incubate them with a biotin-conjugated secondary antibody and Avidin conjugated with Horseradish Peroxidase.
Incubate for the required time. The blue colour is caused by an enzyme reaction that HRP catalyzes. This can be done by washing the plate as per the protocol. The next step is to add the Stop Solution. This will stop the HRP reaction. The blue colour changes to yellow when the signal intensity is measured using a plate reader at 425nm. You can measure the amount of bound Human Hepc in your samples by using the kit assay.